Coding

Part:BBa_K511700

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-27)


TEV Protease MammoBlock

This part encodes a the N1a catalytic proteolytic domain of the tobacco etch virus (TEV). This domain recognizes the amino acid sequence ENLYFQX (where X is a variable amino acid) with unparalleled specificity, and is typically used for the removal of tags made for protein purification. This sequence has been codon-optimized for use in mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 211
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 211
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 211
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 211
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 591


[edit]
Categories
Parameters
n/aTEV Protease MammoBlock